Scientific Staff Member (Structural Biology) | Biologe (m/w/d)
Universität Tuebingen
Job Summary
This permanent, part-time (50%) Scientific Staff Member position at the University of Tübingen focuses on cutting-edge research in Dynamic Structural Biology, specifically the biophysical characterization of nucleic acid-protein complexes and chromatin-based systems. The successful candidate will be responsible for preparing and purifying complex, fluorophore-labeled nucleic acid and nucleosome substrates, conducting quality control on FRET-labeled libraries, and applying advanced biochemical and biophysical assays to functionally characterize chromatin remodelers. A core requirement is extensive practical expertise in High-Throughput Single-Molecule FRET (smFRET) microscopy, including modern multiplexing techniques like MUSCLE technology. The role requires a completed Ph.D. in a relevant natural science field, strong analytical skills for complex data sets, and the ability to work independently within an interdisciplinary research environment.
Required Skills
Education
Degree in Molecular Biology, Biochemistry, or equivalent natural science discipline; Completed Ph.D.
Experience
- Long-term experience in preparation and purification of complex, fluorophore-labeled nucleic acid and nucleosome substrates for single-molecule microscopy
- Professional experience in manufacturing and quality control of FRET-labeled nucleic acid and nucleosome libraries
- Professional experience in applying biochemical and biophysical assays for functional characterization of chromatin remodelers
- Extensive practical knowledge in High-Throughput Single-Molecule FRET (smFRET) microscopy, including modern multiplexing approaches
- Proven experience in experimental data acquisition and quantitative data evaluation and analysis of complex smFRET datasets
Languages
Additional
- Ability for independent scientific work; Ability for close collaboration within an interdisciplinary research team.